Sticky ends from different EcoO109I sites may not be compatible.Įfficient cleavage requires at least two copies of the NgoMIV recognition sequence.Įfficient cleavage requires at least two copies of the NaeI recognition sequence. Sticky ends from different BlpI sites may not be compatible. PaeR7I does not recognize the sequence CTCTCGAG. Sticky ends from different BseRI sites may not be compatible.īsoBI is typically used at 37☌, but can be used at temperatures up to 65☌. G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BtgI sites may not be compatible. Prolonged incubation with NdeI may lead to removal of additional nucleotides.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Sticky ends from different AhdI sites may not be compatible.īsaHI is typically used at 37☌, but is even more active at 60☌.
0 kommentar(er)
